Target (protein/gene name): GlcB, malate synthase G E.C Number: 2.3.3.9 NCBI Gene # or RefSeq#: NC_002677.1 PBD ID: 4EX4 Organism: Mycobacterium leprae (strain Br4923) Etiologic Risk Group: n/a Disease Information: Mycobacterium Leprae is a slow-growing bacteria that causes Leprosy, which is also known as Hansen's Disease. Leprosy is a human disease that affects the skin, peripheral nerves, eyes, and mucosa of the upper respiratory tract. The bacteria causes numbness, nerve damage, and gradual tissue decay and disfigurement of the limbs. The nerve damage from the disease can lead paralysis and crippled hands and feet. The signs and symptoms of leprosy mainly affect the skin, nerves, and mucous membranes. Some signs are discolored skin patches, loss of eyebrow hair or eyelashes, muscle weakness or paralysis, enlarged nerves, etc.
Essentiality: Malate synthase catalyzes the irreversible condensation of acetyl-CoA with glyoxylate which forms (S)-malate. Also, the enzyme plays a role in the glyoxylate cycle, which leads to the loss of CO(2).
Assayed by adding cell extracts to H2O2 in K2HPO4. O-dianisidine was used for peroxidase and was added to a phosphate buffer and H2O2 in deionized water.
Materials and Reagents:
The enzyme catalyses the irreversible condensation of acetyl-CoA with glyoxylate to form (S)-malate. Among other functions, the enzyme participates in the glyoxylate cycle, a modified version of the TCA cycle that bypasses steps that lead to a loss of CO2.
Query Coverage: 100% Max % Identities: 100% % Positives: 100% Chain used for Homology: A Similarity to Human: No significant similarity was found to show any results Inhibitors: None Expression:
Malate synthase in mycobacterium leprae is expressed and causes the disease Leprosy. Transmembrane Bound Regions: There are 3-4 transmembrane bound regions. Fig 2. TMPred output graph for Malate Synthase
E.C Number: 2.3.3.9
NCBI Gene # or RefSeq#: NC_002677.1
PBD ID: 4EX4
Organism: Mycobacterium leprae (strain Br4923)
Etiologic Risk Group: n/a
Disease Information: Mycobacterium Leprae is a slow-growing bacteria that causes Leprosy, which is also known as Hansen's Disease. Leprosy is a human disease that affects the skin, peripheral nerves, eyes, and mucosa of the upper respiratory tract. The bacteria causes numbness, nerve damage, and gradual tissue decay and disfigurement of the limbs. The nerve damage from the disease can lead paralysis and crippled hands and feet. The signs and symptoms of leprosy mainly affect the skin, nerves, and mucous membranes. Some signs are discolored skin patches, loss of eyebrow hair or eyelashes, muscle weakness or paralysis, enlarged nerves, etc.
Links:
1.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076554/
2. https://www.cdc.gov/leprosy/index.html
Essentiality: Malate synthase catalyzes the irreversible condensation of acetyl-CoA with glyoxylate which forms (S)-malate. Also, the enzyme plays a role in the glyoxylate cycle, which leads to the loss of CO(2).
1. https://enzyme.expasy.org/cgi-bin/enzyme/enzyme-search-ec
Druggable Target: Not available
Assayable Enzyme Target:http://jcm.asm.org/content/20/3/421.full.pdf
Assayed by adding cell extracts to H2O2 in K2HPO4. O-dianisidine was used for peroxidase and was added to a phosphate buffer and H2O2 in deionized water.
Materials and Reagents:
The enzyme catalyses the irreversible condensation of acetyl-CoA with glyoxylate to form (S)-malate. Among other functions, the enzyme participates in the glyoxylate cycle, a modified version of the TCA cycle that bypasses steps that lead to a loss of CO2.
Link to Brenda: https://www.brenda-enzymes.org/enzyme.php?ecno=2.3.3.9
Structure:
Image of Protein:
Fig 1. Structure of GlcB from Mycobacterium leprae
Pairwise alignment of BlastP:
<span style="background-color: #ffffff; color: #222222; font-size: 11.869px;">Query 1 ATGACTGATCGCGTGTCGGCGGGGAATTTGCGCGTCGCCCGTGTGCTTTACGACTTCGTC 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110146 ATGACTGATCGCGTGTCGGCGGGGAATTTGCGCGTCGCCCGTGTGCTTTACGACTTCGTC 110205 Query 61 AACAACGAAGCCCTGCCTGGCACCGACATCAACCCAAACAGCTTCTGGTCGGGCGTCGCC 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110206 AACAACGAAGCCCTGCCTGGCACCGACATCAACCCAAACAGCTTCTGGTCGGGCGTCGCC 110265 Query 121 AAGGTCGTCGCCGACCTCACCCCGCAAAACCAAAGTTTGCTGAACTCCCGCGATGAGCTG 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110266 AAGGTCGTCGCCGACCTCACCCCGCAAAACCAAAGTTTGCTGAACTCCCGCGATGAGCTG 110325 Query 181 CAGGCCCAGATTGACAAATGGCACCGCCATCGTGTGATCGAGCCATTCGACGTGGATGCT 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110326 CAGGCCCAGATTGACAAATGGCACCGCCATCGTGTGATCGAGCCATTCGACGTGGATGCT 110385 Query 241 TATCGGCAGTTCCTCATCGATATCGGCTATCTGCTGCCTGAACCCGATGACTTCACCATC 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110386 TATCGGCAGTTCCTCATCGATATCGGCTATCTGCTGCCTGAACCCGATGACTTCACCATC 110445 Query 301 AGCACCTCAGGAGTAGACGACGAGATCACCATGACCGCCGGCCCGCAGCTGGTGGTGCCG 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110446 AGCACCTCAGGAGTAGACGACGAGATCACCATGACCGCCGGCCCGCAGCTGGTGGTGCCG 110505 Query 361 GTACTTAACGCACGGTTCGCACTCAACGCCGCCAACGCCCGCTGGGGCTCCCTTTACGAC 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110506 GTACTTAACGCACGGTTCGCACTCAACGCCGCCAACGCCCGCTGGGGCTCCCTTTACGAC 110565 Query 421 GCTCTATATGGCACCGACACCATACCGGAAACCGAGGGAGCCGAAAAGGGCTCTGAGTAC 480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110566 GCTCTATATGGCACCGACACCATACCGGAAACCGAGGGAGCCGAAAAGGGCTCTGAGTAC 110625 Query 481 AACAAGATTCGCGGCGACAAGGTGATCGCCTACGCACGCAAGTTTATGGATCAGGCCGTC 540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110626 AACAAGATTCGCGGCGACAAGGTGATCGCCTACGCACGCAAGTTTATGGATCAGGCCGTC 110685 Query 541 CCGCTGGCTTCTGACTCATGGACTAACGCTACGGGAGTGAGCATCTTCGACGGCCAGCTT 600 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110686 CCGCTGGCTTCTGACTCATGGACTAACGCTACGGGAGTGAGCATCTTCGACGGCCAGCTT 110745 Query 601 CAGATCGCCATCGGCACGAATTCCACCGGATTAGCCAGTCCCGAAAAGTTTGTCGGCTAC 660 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110746 CAGATCGCCATCGGCACGAATTCCACCGGATTAGCCAGTCCCGAAAAGTTTGTCGGCTAC 110805 Query 661 AATCGCCAGTTGCGATCCTCCAACTGGTCGGTGCTGTTGGCCAATCACGGCCTGCACATC 720 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110806 AATCGCCAGTTGCGATCCTCCAACTGGTCGGTGCTGTTGGCCAATCACGGCCTGCACATC 110865 Query 721 GAGGTCTTGATCGACCCGGAATCACCAATAGGCAAGACTGACCCCGTCGGCATCAAGGAT 780 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110866 GAGGTCTTGATCGACCCGGAATCACCAATAGGCAAGACTGACCCCGTCGGCATCAAGGAT 110925 Query 781 GTGATCCTGGAATCGGCCATCACCACGATCATGGACTTCGAAGACTCAGTCACTGCCGTC 840 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110926 GTGATCCTGGAATCGGCCATCACCACGATCATGGACTTCGAAGACTCAGTCACTGCCGTC 110985 Query 841 GACGCCGACGACAAGGTACGCGGCTACCGCAACTGGCTCGGCCTGAACAAAGGTGACTTA 900 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110986 GACGCCGACGACAAGGTACGCGGCTACCGCAACTGGCTCGGCCTGAACAAAGGTGACTTA 111045 Query 901 ACCGAGGAAGTCAACAAGGACGGCAAGACCTTCACCCGCGTGCTCAACGCCGACCGCAGC 960 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111046 ACCGAGGAAGTCAACAAGGACGGCAAGACCTTCACCCGCGTGCTCAACGCCGACCGCAGC 111105 Query 961 TACACCACGCCCGACGGCGGTGAATTGACCCTGCCCGGGCGCAGCCTGTTGTTCGTCCGC 1020 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111106 TACACCACGCCCGACGGCGGTGAATTGACCCTGCCCGGGCGCAGCCTGTTGTTCGTCCGC 111165 Query 1021 AACGTGGGCCATCTGACGACCAGCGACGCGATTCTCGTGGACGGAGGTGACGGTCAGGAA 1080 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111166 AACGTGGGCCATCTGACGACCAGCGACGCGATTCTCGTGGACGGAGGTGACGGTCAGGAA 111225 Query 1081 AAAGAAGTGTTCGAGGGCATCATAGATGCAGTGTTCACCGGTCTGGCCGCAATCCACGGA 1140 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111226 AAAGAAGTGTTCGAGGGCATCATAGATGCAGTGTTCACCGGTCTGGCCGCAATCCACGGA 111285 Query 1141 CTCAAAACCGGCGAAGCTAATGGCCCACTGACCAACAGCCGCACCGGTTCCATCTACATC 1200 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111286 CTCAAAACCGGCGAAGCTAATGGCCCACTGACCAACAGCCGCACCGGTTCCATCTACATC 111345 Query 1201 GTCAAGCCGAAAATGCACGGTCCCGCGGAGGTTGCGTTCACTTGCGAGCTGTTCAGCCGC 1260 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111346 GTCAAGCCGAAAATGCACGGTCCCGCGGAGGTTGCGTTCACTTGCGAGCTGTTCAGCCGC 111405 Query 1261 GTCGAAGACGTGCTGGGGTTGCCGCAGGGCACCTTGAAGGTCGGCATCATGGATGAGGAG 1320 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111406 GTCGAAGACGTGCTGGGGTTGCCGCAGGGCACCTTGAAGGTCGGCATCATGGATGAGGAG 111465 Query 1321 CGGCGTACGACACTCAATTTGAAGGCGTGCATCAAGGCCGCCGCCGATCGGGTCGTGTTT 1380 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111466 CGGCGTACGACACTCAATTTGAAGGCGTGCATCAAGGCCGCCGCCGATCGGGTCGTGTTT 111525 Query 1381 ATCAACACTGGCTTCTTGGACCGCACCGGCGACGAGATCCACACCTCAATGGAGGCCGGC 1440 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111526 ATCAACACTGGCTTCTTGGACCGCACCGGCGACGAGATCCACACCTCAATGGAGGCCGGC 111585 Query 1441 CCGATGATTCGTAAGGGTGCGATGAAAAACAGCACCTGGATCAAAGCCTACGAGGACGCC 1500 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111586 CCGATGATTCGTAAGGGTGCGATGAAAAACAGCACCTGGATCAAAGCCTACGAGGACGCC 111645 Query 1501 AATGTTGACATCGGACTAGCCGCAGGCTTCAAGGGCAAAGCACAGATCGGCAAGGGCATG 1560 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111646 AATGTTGACATCGGACTAGCCGCAGGCTTCAAGGGCAAAGCACAGATCGGCAAGGGCATG 111705 Query 1561 TGGGCAATGACCGAATTAATGGCTGATATGGTCGAGCAGAAGATCGGCCAGCCTAAGGCG 1620 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111706 TGGGCAATGACCGAATTAATGGCTGATATGGTCGAGCAGAAGATCGGCCAGCCTAAGGCG 111765 Query 1621 GGCGCCACCACCGCGTGGGTACCCTCGCCCACTGCGGCCACGCTGCATGCGATGCACTAC 1680 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111766 GGCGCCACCACCGCGTGGGTACCCTCGCCCACTGCGGCCACGCTGCATGCGATGCACTAC 111825 Query 1681 CACCAGGTCGACGTGGCTGCCGTGCAACAAGAACTGACCGGCCAGAGACGTGCCACCGTT 1740 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111826 CACCAGGTCGACGTGGCTGCCGTGCAACAAGAACTGACCGGCCAGAGACGTGCCACCGTT 111885 Query 1741 GACCAGCTGTTGACCATTCCGTTGGCCAAGAAACTGGCCTGGGCTCCCGAGGAGATCCGC 1800 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111886 GACCAGCTGTTGACCATTCCGTTGGCCAAGAAACTGGCCTGGGCTCCCGAGGAGATCCGC 111945 Query 1801 GAGGAGGTCGATAATGACTGTCAGTCCATCCTCGGTTATGTGGTGCGCTGGGTGGACCAG 1860 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111946 GAGGAGGTCGATAATGACTGTCAGTCCATCCTCGGTTATGTGGTGCGCTGGGTGGACCAG 112005 Query 1861 GGTATCGGTTGCTCGAAGGTGCCTGACATCCACAATGTGGCACTAATGGAGGACCGCGCC 1920 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112006 GGTATCGGTTGCTCGAAGGTGCCTGACATCCACAATGTGGCACTAATGGAGGACCGCGCC 112065 Query 1921 ACCCTGCGGATCTCGAGCCAGTTGCTAGCAAACTGGCTGCGCCACGGCGTAATCACCAGC 1980 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112066 ACCCTGCGGATCTCGAGCCAGTTGCTAGCAAACTGGCTGCGCCACGGCGTAATCACCAGC 112125 Query 1981 GAGGACGTGCGGGCCAGCCTGGAACGGATGGCGCCGTTAGTCGATCAGCAAAACGCCGAA 2040 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112126 GAGGACGTGCGGGCCAGCCTGGAACGGATGGCGCCGTTAGTCGATCAGCAAAACGCCGAA 112185 Query 2041 GACCCAGCATATCGCCCGATGGCGCCTAATTTCGATGACAGCATCGCGTTCCTAGCGGCG 2100 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112186 GACCCAGCATATCGCCCGATGGCGCCTAATTTCGATGACAGCATCGCGTTCCTAGCGGCG 112245 Query 2101 CAGGAACTGATACTGTCCGGCGCACAACAGCCCAACGGCTATACCGAGCCGATCCTGCAT 2160 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112246 CAGGAACTGATACTGTCCGGCGCACAACAGCCCAACGGCTATACCGAGCCGATCCTGCAT 112305 Query 2161 CGCCGTCGTCGCGAATTCAAGGCACAGAACCGGTGA 2196 |||||||||||||||||||||||||||||||||||| Sbjct 112306 CGCCGTCGTCGCGAATTCAAGGCACAGAACCGGTGA 112341 </span>Query Coverage: 100%Max % Identities: 100%
% Positives: 100%
Chain used for Homology: A
Similarity to Human: No significant similarity was found to show any results
Inhibitors: None
Expression:
Malate synthase in mycobacterium leprae is expressed and causes the disease Leprosy.
Transmembrane Bound Regions: There are 3-4 transmembrane bound regions.
Fig 2. TMPred output graph for Malate Synthase
Gene Available:
https://www.atcc.org/Products/All/4243.aspx
Gene Sequence: